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protein dbp  (Athens Research)


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    Athens Research protein dbp
    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin <t>or</t> <t>Fibronectin</t> are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein <t>(DBP)</t> are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
    Protein Dbp, supplied by Athens Research, used in various techniques. Bioz Stars score: 95/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Circulatory proteins shape microglia state and boost phagocytosis"

    Article Title: Circulatory proteins shape microglia state and boost phagocytosis

    Journal: bioRxiv

    doi: 10.1101/2024.09.30.615861

    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin or Fibronectin are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein (DBP) are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
    Figure Legend Snippet: Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin or Fibronectin are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein (DBP) are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.

    Techniques Used: Flow Cytometry, Labeling, Injection, Saline, Binding Assay



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    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin <t>or</t> <t>Fibronectin</t> are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein <t>(DBP)</t> are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
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    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin <t>or</t> <t>Fibronectin</t> are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein <t>(DBP)</t> are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
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    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin <t>or</t> <t>Fibronectin</t> are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein <t>(DBP)</t> are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
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    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin <t>or</t> <t>Fibronectin</t> are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein <t>(DBP)</t> are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
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    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin <t>or</t> <t>Fibronectin</t> are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein <t>(DBP)</t> are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
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    Image Search Results


    Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin or Fibronectin are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein (DBP) are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.

    Journal: bioRxiv

    Article Title: Circulatory proteins shape microglia state and boost phagocytosis

    doi: 10.1101/2024.09.30.615861

    Figure Lengend Snippet: Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin or Fibronectin are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein (DBP) are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.

    Article Snippet: ApoA-I protein (Athens Research &Technology, 16-16-120101-LEL), ApoA-II (Athens Research & Technology, 16-16-120102), ApoM (LSbio, LS-G14868), ApoE (Sigma, SRP4760), Albumin (Sigma, 126674), Clusterin (Sino Biological, 50485), Fibrinogen (Abcam, ab92791), Vitronectin (Sino Biological, 50585-M08H), Fibronectin (Corning, 354008), Plasminogen (Innovative research, IMSPLG1MG), Vitamin D-binding protein (DBP) (Athens Research &Technology, 16-16-070307), and Transferrin (Sigma, T0665) were used for labeling.

    Techniques: Flow Cytometry, Labeling, Injection, Saline, Binding Assay

    Materials

    Journal: Theranostics

    Article Title: Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

    doi: 10.7150/thno.90322

    Figure Lengend Snippet: Materials

    Article Snippet: Mouse monoclonal anti-Vitamin D binding protein (DBP) , , Proteintech , CAT#66175-1-Ig , RRID: AB_2881570.

    Techniques: Luciferase, Reporter Gene Assay, Silver Staining, Binding Assay, Molecular Weight, In Situ, Membrane, Magnetic Beads, Transfection, Modification, H&E Stain, Staining, cDNA Synthesis, SYBR Green Assay, Bicinchoninic Acid Protein Assay, In Vivo, Plasmid Preparation, CRISPR, Knock-Out, Sequencing, Software, Imaging